Table 3. Effect of CCCP treatment on the survival of granulosa cells and mtDNA copy number in granulosa cells and spent culture medium.

Group	No. of trials	Cell number in well	Survival rate (%)	Annexin positive (%)	Relative value of LDH	mtDNA in granulosa cell	mtDNA in medium (/100 μl)
Vehicle	8	143125 ± 6186	91.6 ± 0.1	5.4 ± 0.2	1.0 ± 0.02	590.7 ± 25.3	12,494.6 ± 668.8 a
CCCP	8	137000 ± 5463	88.3 ± 2.5	5.1 ± 0.2	0.96 ± 0.00	545.9 ± 31.6	15,005.1 ± 905.7 b

Data are represented as mean ± SEM value. Average LDH values of control are defined as 1.0. a–b: P < 0.05. Mitochondrial DNA copy number (mtDNA) was determined by real-time PCR targeting mitochondrial short sequences.