Table 1. Development of rat embryos introduced to ZFN and TALEN mRNA by microinjection or electroporation.
| mRNA | Methods | Pulse width (ms) |
No. of embryos examined |
No. (%) of 2-cell embryos |
No. (%) of offspring |
No. (%) of offspring with mutation |
|---|---|---|---|---|---|---|
| ZFN | Microinjection | – | 93 | 41 (44) | 9 (10) | 3 (33) |
| Electroporation | 0.5 | 61 | 58 (95) | 19 (31) | 7 (37) | |
| 1.5 | 63 | 57 (91) | 15 (24) | 11 (73) | ||
| 2.5 | 66 | 16 (24) | 4 (6) | 3 (75) | ||
| TALEN | Microinjection | – | 52 | 20 (39) | 6 (12) | 6 (100) |
| Electroporation | 1.5 | 57 | 55 (97) | 25 (44) | 1 (4) | |
| 2.5 | 57 | 56 (98) | 17 (30) | 3 (18) | ||