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| Cas9 mRNA (μg/ml) |
gRNA (μg/ml) |
ssODN (μg/ml) |
No. of embryos examined |
No. (%) of embryos developed to 2-cells |
No. (%) of males offspring |
No. (%) of knockout offspring |
No. (%) of knock-in offspring |
|---|---|---|---|---|---|---|---|
| 400 | 600 | 300 | 60 | 45 (75) | 24 (53) | 21 (88) | 8 (33) |
| 200 | 200 | 200 | 50 | 49 (98) | 19 (39) | 7 (37) | 1 (5) |
| 100 | 100 | 100 | 89 | 88 (99) | 41 (47) | 16 (39) | 1 (2) |
