Table 1. Sequences of primers and annealing conditions used for quantitative PCR analyses.

Gene symbol	Entrez number	Amplicon location (bp)	Primer sets	Primer conc. (nM)	Annealing temp. (ºC)
ATP5O	281640	46−146	5’-TTGACCTACAGCTGCCAGGAG	600	57
			3’-TTGGCAAATGGCCTGACCACAG
		340−452	5’-GCCTAAGTGACATGACGGCA	600	61
			3’-AATGCGGAAATCACCGCAGG
COQ3	540298	114−244	5’-AACTACGTCGGCGGTTTATCC	200	57
			3’-TCATCCAATTCAGGCAGGCA
		664−761	5’-ACGTTTGATGCTGTCGTAGC	200	56
			3’-GAACCATCAGGCTTTAACAC
NDUFC2	338046	145−329	5’-CGTCACAATGATGACTGGACGGCA	200	60
			3’-GATGCAAACCGGCCAACAGCACC
		397−518	5’-GTATGCTGTGAGGGACCACG	200	57
			3’-TACTTCAACGCACTGGATGG
GFP 1	–	–	5’-CAACTTCAAGACCCGCCACA	800	–
			3’-TCTGGTAAAAGGACAGGGCCA

¹ Referenced primer set from [11].