Table 2. Top enriched functional categories and associated transcripts altered at 24 hIVM in oocytes by heat stress exposure for the first 12 hIVM 1.
| Annotation cluster & members 2 | Enrichment score | Gene count | P-value | Genes 3 |
|---|---|---|---|---|
| Cluster 1 | 1.93 | SNRPB2↓, LSM6↓, PPIH↓, SNROC↓, TXNL4A↓, SRSF5↓ | ||
| Spliceosome (KEGG) | 6 | < 0.01 | ||
| U4/U6 x U5 tri-snRNP complex (CC) | 3 | < 0.01 | ||
| Spliceosomal complex (CC) | 3 | < 0.05 | ||
| Cluster 2 | 1.89 | RNF144B↓, ATP5O↓, ADHFE1↓, COQ3↓, COQ5↓, MRPS17↓, PPIF↓, MRPL2↓, ABCG2↓, MRPL1↓, NDUFC2↓, NDUFC1↓, NIT1↓ | ||
| Mitochondrion (UP) | 13 | < 0.01 | ||
| Transit peptide (UP) | 8 | < 0.01 | ||
| Transit peptide:mitochondrion (UP) | 8 | < 0.05 | ||
| Mitochondrion inner membrane (UP) | 5 | < 0.05 | ||
| Cluster 3 | 1.48 | RNF144B↓, VPS41↓, RAD18↓, RNF170↓, RNF166↓, MNAT1↓, RNF139↓, BHMT↓, WT1P↓, DDX58↓, ANF593↓, SNRPC↓ | ||
| Zinc finger, RING-type (IP) | 7 | < 0.01 | ||
| RING (SM) | 6 | < 0.05 | ||
| Zinc finger, RING-type, conserved site (IP) | 5 | < 0.05 | ||
| Zinc ion binding (MF) | 12 | < 0.05 | ||
| Zinc finger, RING/FYVE/PHD-type (IP) | 7 | < 0.05 | ||
| Ubiquitin protein ligase activity (MF) | 4 | < 0.10 | ||
| Protein polyubiquitination (BP) | 3 | < 0.10 | ||
| Cluster 4 | 1.28 | ATP5O↓, ATP5L↓, ATP6V0D1↓, NDUFC2↓, COX6A1↑, NDUFC1↓ | ||
| Oxidative phosphorylation (KEGG) | 6 | < 0.01 | ||
| Parkinson’s disease (KEGG) | 5 | < 0.05 | ||
| Mitochondrion inner membrane | 5 | < 0.05 | ||
| Huntington’s disease (KEGG) | 5 | < 0.10 | ||
| Alzheimer’s disease (KEGG) | 4 | > 0.05 | ||
| Non-alcoholic fatty liver disease (KEGG) | 3 | > 0.05 | ||
1 Heat stress (41.0°C) applied only during first 12 h of in vitro maturation (hIVM), followed by incubation at control (38.5°C) temperature for remaining 12 hIVM. 2 DAVID category terms: BP, biological function; CC, cellular component; IP, Interpro; KEGG, Kyoto Encyclopedia of Genes and Genomes pathway; MF, molecular function; UP, UniProt. 3 ↑ Levels increased by heat stress, ↓ Levels decreased by heat stress.