Table 6. Embryo development of oocytes matured at 38.5 or 41.0°C 1.
| IVM temperature (°C) |
Cleavage stage development | Blastocyst development | |||||
|---|---|---|---|---|---|---|---|
| (72 h post-IVF) |
(204 to 216 h post-IVF) |
||||||
| Cleaved | 2-Cell 3 | 4-Cell 3 | 8 to 16-Cell 3 | Percent of PZ | Stage | Quality | |
| (%) | (%) | (%) | (%) | ||||
| 38.5 | 70.05 | 8.72 | 22.02 b | 71.00 a | 29.73 a | 6.80 | 1.51 |
| 41.0 | 69.87 | 9.22 | 27.08 a | 63.50 b | 19.91 b | 6.89 | 1.59 |
| Pooled SEM | 2.58 | 1.44 | 1.38 | 2.42 | 2.26 | 0.09 | 0.07 |
| P-value | > 0.05 | > 0.05 | < 0.05 | < 0.05 | < 0.001 | > 0.05 | > 0.05 |
IVM, in vitro maturation; PZ, presumptive zygote. 1 First 12 h of maturation followed by 38.5°C thereafter. 2 Number of presumptive zygotes evaluated for cleavage. 3 Relative to proportion of embryos cleaved. 4 Number of presumptive zygotes evaluated for blastocyst development only (a different subset than those for cleavage). ab Means differ within a column (P < 0.05).