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Schematic representation of the experimental design. COCs were cultured in IVM medium with/without 0.1 IU/ml FSH or 100 ng/ml AREG for 22 h (Standard IVM). As another experimental group, COCs were cultured in IVM medium with/without FSH and AREG after pre-IVM with/without 1 mM dbcAMP and 500 μM IBMX for 2 h (Pre-IVM).
