Fig. 5.

Effect of cAMP-modulated pre-IVM on expression of EGFR and FSHR mRNA in cumulus cells. Expression of EGFR (A) and FSHR (B) in cumulus cells from COCs before pre-IVM (0 h) or after no treatment (control) or treatment with dbcAMP and/or IBMX for 2 h. Gene expression was normalized to expression of the housekeeping gene RPL19. The value at 0 h was set as one and all other points are expressed relative to this point. Values represent mean ± SEM of five replicates. Bars with no common superscript letters indicate significant differences between treatments at P < 0.05.