Fig. 6.

Regulation of oocyte-cumulus cells/cumulus cells-cumulus cells gap-junctional communication in COCs with standard IVM or pre-IVM conditions. LY diffusion of COCs cultured in IVM medium (Standard IVM) or in IVM medium after pre-IVM without (control) or with dbcAMP and/or IBMX for 2 h (Pre-IVM) was examined at 0, 3, and 6 h of in vitro oocyte maturation, respectively. No ligand was included in the IVM medium. Values represent mean ± SEM of five replicates. Points with different letters within a time-point indicate significant differences between treatments at P < 0.05.