Fig. 7.

Effect of EGFR signaling inhibition on developmental competence of oocytes matured following cAMP-modulated pre-IVM. COCs were treated with dbcAMP and IBMX for 2 h and then cultured in IVM medium supplemented with FSH or AREG, either in the absence (–AG) or presence of 10 μM of the EGFR tyrosine kinase inhibitor, AG1478 (+AG). Developmental competence was examined by measuring embryo development at the following time points: cleaved embryos (A), > 4-cell embryos (B) at day 2 and embryos developed to the blastocyst (C) and expanded blastocyst stages (D) at day 7. Blastocyst and expanded blastocyst formation rates are expressed as a percentage of cleaved embryos. Values represent means ± SEM of five replicates. Bars with no common superscript letters indicate significant differences at P < 0.05.