Table 2. Comparison of pre-IVM with dbcAMP versus forskolin on oocyte developmental competence.
| IVM protocol | No. of cultured embryo (replicates) |
Percentage of embryos (mean ± SEM%) |
|||
|---|---|---|---|---|---|
| Cleavage |
Blastocyst / Cleavage |
||||
| Total | 4 cells < | Total | Expanded stage | ||
| Standard IVM * | 91 (5) | 86.7 ± 2.5 | 67.9 ± 4.6 | 40.4 ± 2.2 b | 29.0 ± 1.9 b |
| Pre-IVM with dbcAMP+IBMX ** | 96 (5) | 90.5 ± 2.1 | 70.6 ± 3.8 | 62.4 ± 4.9 a | 52.1 ± 5.5 a |
| Pre-IVM with FSK+IBMX *** | 82 (5) | 80.7 ± 3.1 | 64.5 ± 6.1 | 53.2 ± 2.4 a | 32.4 ± 3.4 ab |
* Cumulus oocyte complexes (COCs) were cultured in FSH-supplemented IVM medium for 22 h. ** COCs were treated with 1 mM dbcAMP and 500 μM IBMX for 2 h and then cultured in FSH-supplemented IVM medium for 22 h. *** COCs were treated with 100 μM forskolin (FSK) and 500 μM IBMX for 2 h and then cultured in FSH-supplemented IVM medium for 22 h. a,b Values are significantly different among IVM protocol (P < 0.05).