FIG 5.

Antiviral activity of PF-06409577 against WNV, ZIKV, and DENV-2. (A) Dose-response curves for Vero cells infected with WNV, ZIKV, and DENV-2 (MOI of 1 PFU/cell) and treated with increasing amounts of PF-06409577. Virus yield in supernatant was determined at 24 h p.i. by plaque assay (n = 4). (B) Vero cells were treated with increasing amounts of PF-06409577, and the cellular ATP content was determined as an estimation of cell viability at 24 or 48 h posttreatment (n = 4). Dashed lines denote a 50% reduction. Data are expressed as mean ± SD.