TABLE 1.
Selectivity indexes of PF-06409577 against WNV, ZIKV, and DENV-2
| Variable | Virus | PF-06409577 values after treatment for: |
|
|---|---|---|---|
| 24 h | 48 h | ||
| CC50a (μg/ml [μM]) | 315.7 (923.7) | 201.4 (586.4) | |
| EC50b (μg/ml [μM]) | WNV | 8.2 (24.1) | |
| ZIKV | 0.9 (2.6) | ||
| DENV-2 | 4.6 (13.4) | ||
| SI (CC50/EC50) | WNV | 38.5 | |
| ZIKV | 350.8 | ||
| DENV-2 | 43.4 | ||
a
The half-maximal cytotoxic concentration (CC50) is the concentration that results in the reduction of 50% of the amount of cellular ATP after 24 or 48 h of treatment of uninfected cells.
b
The half-maximal effective concentration (EC50) is the concentration of drug at which virus yield (MOI of 1 PFU/cell) is inhibited by 50%. Virus yield was determined by plaque assay 24 h p.i. for WNV and ZIKV and 48 h p.i. for DENV-2.