FIG 4.

Alignment of MDA3349 DHPS (FolP) and DHPS2 (FolP2). Blue and orange residues correspond to absolutely conserved and highly conserved residues, respectively (46). The two proteins share 36% sequence identity. Residues highlighted in yellow were found to be mutated in laboratory-adapted Nocardia strains (for mutations in strains other than MDA3349, the mutated residues of the other strains are shown using the numbering of MD3349). “*” represents conserved residues. “:” represents amino acids with strongly similar properties. “.” represents amino acids with weakly similar properties.