FIG 6.

Simeprevir sensitivities of the NS3 variants containing Q80R with various amino acid mutations at position 155. HCV RNAs encoding NS3 variants, such as Q80R+R155K, Q80R+R155T, Q80R+R155G, Q80R+R155W, or Q80R+R155Q in H77S.3/GLuc2A, were transfected into Huh-7.5 cells. The medium was replaced with fresh medium containing only DMSO or simeprevir at 100, 250, 500, 750, 1,000, and 5,000 nM after 24 h and at 24-h intervals thereafter. The secreted GLuc activity in the medium was determined at 72 h posttransfection. For each NS3 variant, the GLuc activity in simeprevir-treated cells was normalized to that in cells treated with DMSO only.