Decrease in absorbance after incubation with amoxicillin. Absorbance was measured at 562 (corrected for absorbance at 630 nm), and the decrease in absorbance was measured after 72 h of incubation with amoxicillin and compared with the decrease in absorbance of the positive-control sample ([Et0 − Et72] of <25% of [EPOS,t0 − EPOS,t72]). Shown are representative results of two individual experiments with five representative Borrelia strains, i.e., PKo (B. afzelii), N40 (B. burgdorferi
sensu stricto), HS1 (B. hermsii from the United States), and Izh-14 and Yek-1 (B. miyamotoi clinical isolates). Symbols and error bars represent the mean ± standard error of the mean of quadruplicate results. If no error bars are visible, then the error bars were smaller than the symbol.