TABLE 2.
Plasmids used in the study
| Plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| pJET1.2/blunt | rep(pMB1), blaR eco47IR, PlacUV5 | Thermo Fisher Scientific |
| U-pJET | pJET1.2/blunt with 1 kb upstream of >atl gene of S. aureus MW2 | This study |
| D-pJET | pJET1.2/blunt with 1 kb downstream of atl gene of S. aureus MW2 | This study |
| UD-pJET | pJET1.2/blunt with upstream and downstream of atl gene of S. aureus MW2 | This study |
| UKD-pJET | pJET1.2/blunt with kanamycin cassette in between of the upstream and downstream of atl gene of S. aureus MW2 | This study |
| pMAD | ermC blaR; oripBR322; pclpB bgaB, oripE194ts | 43 |
| UKD-pMAD | pMAD with the UKD fragment insert | This study |