FIG 6.

Incorporation of unsaturated fatty acids into S. aureus ATCC 27217 bacterial membranes increases daptomycin bactericidal activity, whereas incorporation of stearic acid (C₁₈) decreases the antibiotic activity. (A) Log reduction in bacterial counts after 6 h of daptomycin treatment compared with the initial bacterial concentration. TSBpc+C₁₈ corresponds to stearic acid-supplemented medium, and TSBpc+C_18:1 corresponds to oleic acid-supplemented medium. (B) Proportions of S. aureus ATCC 27217 fatty acids with added oleic acid in medium. Error bars represent the standard deviation based on six experiments.