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Functional assignment of Mur28. (A) HPLC analysis after 12 h of incubation without Mur28 (i) and 12 h of Mur28-catalyzed reaction (ii). Data were collected using GP1. The arrowhead denotes the peak corresponding to D2-PHOS, and the asterisk denotes an unidentified peak. A260, absorbance at 260 nm. (B) Structure of 3″-phospho-muraymycin D2. (C) Comparison of the 1H NMR spectra for muraymycin D2 and the Mur28 product.
