FIG 5.

Microscopic examination of C. hominis infection. (A) Mucosal colonization scores. (B) Mucosal lesion scores. (C) Correlation between colonization scores and lesion scores. The gastrointestinal tissues were fixed in formalin and processed for hematoxylin and eosin staining. Mucosal colonization was examined at a magnification of ×40 and given a score of 0 to 5 (0, no infection detected; 1, 1 to 20% of the epithelial surface infected; 2, 21 to 40% of the surface infected; 3, 41 to 60% of the surface infected; 4, 61 to 80% of the surface infected; and 5, 81 to 100% of the surface infected). Mucosal lesion scores were applied for villus changes, epithelial changes, and cellular inflammation in the lamina propria and within crypts/glands for all samples. The following scores were used: 0, normal; 2.5, equivocal; 5, mild; 10, moderate; and 15, marked. The final lesion score was the sum of scores for 5 anatomic sites (3 for the small intestine [duodenum, jejunum, and ileum] and 2 for the large intestine [cecum and colon]) for each piglet. The scatterplots (A and B) show means ± SEM. The Mann-Whitney test was conducted using GraphPad Prism 7.03. The plot in panel C shows the correlation between scores by linear regression. **, P < 0.01. ●, C. hominis-infected group (n = 9); ■, C. hominis-infected and BKI 1369-treated group (n = 9).