FIG 2.

Disruption of the CypA-NS5A interaction by the cyclophilin inhibitors. The interaction between NS5A-Rluc and CypA-6His was assessed by means of a protein-protein interaction assay with Ni-NTA magnetic beads. After loading of CypA-6His, the beads were incubated with a lysate of Huh7.5 cells expressing WT-NS5A-Rluc (A) or NS5A-D320E-Rluc (B) and two concentrations of C31 and ALV. After washing, CypA-6His-interacting proteins were eluted with imidazole. The NS5A-Rluc activity was measured in the eluate. Huh7.5 cells expressing Rluc were used as a negative interaction control. The data are shown as means ± SD of results from at least three independent experiments. NS, not significant; ***, P < 0.001.