FIG 4.

Amino acid changes selected by serial C31 passages at increasing concentrations in a genotype 1b HCV-SGR. Huh7.5 cells stably harboring a genotype 1b HCV-SGR were cultured in medium containing 1.5 mg/ml of G418 in the presence of increasing doses of C31 until resistant clones were selected. Total RNAs of two resistant clones were extracted, and the NS5A-coding region was sequenced. The amino acid changes observed in the sequences of the NS5A regions spanning domains I to III are shown. Neo, neomycin.