TABLE 2.
C31, ALV, and CsA susceptibility and replication capacity of a wild-type genotype 1b HCV-SGR and effect of introduction of amino acid substitutions selected by serial C31 passages at increasing concentrationsa
| Virus | C31 |
ALV |
CsA |
Mean replication capacity (%) ± SD | |||
|---|---|---|---|---|---|---|---|
| Mean EC50 (μM) ± SD | Fold change | Mean EC50 (μM) ± SD | Fold change | Mean EC50 (μM) ± SD | Fold change | ||
| WT | 2.95 ± 0.60 | 1.0 | 0.03 ± 0.01 | 1.0 | 0.17 ± 0.02 | 1.0 | 100 |
| I133V | 2.75 ± 0.20 | 0.9 | 0.02 ± 0.001 | 0.9 | 0.21 ± 0.04 | 1.2 | 110 ± 23 |
| L183P | NA | NA | NA | NA | NA | NA | 2 ± 1 |
| L303P | 3.05 ± 0.40 | 1.0 | 0.04 ± 0.01 | 1.3 | 0.23 ± 0.04 | 1.4 | 57 ± 7 |
| R304W | 2.47 ± 0.20 | 0.8 | 0.03 ± 0.004 | 1.1 | 0.21 ± 0.004 | 1.2 | 91 ± 4 |
| K308I | 2.59 ± 0.20 | 0.9 | 0.04 ± 0.004 | 1.3 | 0.17 ± 0.03 | 1.0 | 103 ± 3 |
| D320E | 8.73 ± 1.10 | 3.0 | 0.12 ± 0.05 | 4.4 | 0.51 ± 0.04 | 3.0 | 82 ± 16 |
| Y321H | 5.41 ± 0.40 | 1.8 | 0.06 ± 0.02 | 2.2 | 0.41 ± 0.02 | 2.4 | 79 ± 22 |
| E442G | 2.99 ± 0.70 | 1.0 | 0.03 ± 0.004 | 1.1 | 0.24 ± 0.07 | 1.4 | 74 ± 5 |
| L31V/Y93H | 1.20 ± 0.10 | 0.8 | 0.01 ± 0.001 | 0.5 | ND | ND | 92 ± 4 |
a
The data are shown as means ± SD of results from three independent experiments. NA, not available, due to the lack of replication of the mutated HCV-SGR; ND, not done.