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CL attacks N7 of guanines. The single-stranded form of CL2640-d7G, in which the central G is substituted by 7-deaza-G (d7G), was reacted at 37°C with buffer (lanes 1–2) or 100 (lane 3) or 200 µM (lane 4) CL for 24 h (lanes 2–4). After EtOH precipitation samples were further treated with hot piperidine. G+A refers to the purine ladder obtained by treating the wild-type G oligo.
