Table 1.

Oligonucleotide primers used for detection of the virulence genes

Pathogroups	Genes	Sequences	bp	Concentration	References
STEC, EPEC	eae‐F	TCAATGCAGTTCCGTTATCAGTT	482	0.1	Vidal et al. (2005)
	eae‐R	GTAAAGTCCGTTACCCCAACCTG
	escV‐F	ATTCTGGCTCTCTTCTTCTTTATGGCTG	544	0.4	Müller et al. (2007)
	escV‐R	CGTCCCCTTTTACAAACTTCATCGC
	ent‐F	TGGGCTAAAAGAAGACACACTG	629	0.4	Müller et al. (2007)
	ent‐R	CAAGCATCCTGATTATCTCACC
Typical EPEC	bfpB‐F	GACACCTCATTGCTGAAGTCG	910	0.1	Müller et al. (2007)
	bfpB‐R	CCAGAACACCTCCGTTATGC
STEC	hlyEHEC‐F	TTCTGGGAAACAGTGACGCACATA	688	0.1	Antikainen et al. (2009)
	hlyEHEC‐R	TCACCGATCTTCTCATCCCAATG
	stx1A‐F	CGATGTTACGGTTTGTTACTGTGACAGC	244	0.2	Müller et al. (2007)
	stx1A‐R	AATGCCACGCTTCCCAGAATTG
	stx2A‐F	GTTTTGACCATCTTCGTCTGATTATTGAG	324	0.4	Müller et al. (2007)
	stx2A‐R	AGCGTAAGGCTTCTGCTGTGAC
EIEC	ipaH‐F	GAAAACCCTCCTGGTCCATCAGG	437	0.1	Antikainen et al. (2009)
	ipaH‐R	GCCGGTCAGCCACCCTCTGAGAGTAC		0.1	Brandal et al. (2007)
	invE‐F	CGATAGATGGCGAGAAATTATATCCCG	766	0.2	Müller et al. (2007)
	invE‐R	CGATCAAGAATCCCTAACAGAAGAATCAC
EAEC	aggR‐F	ACGCAGAGTTGCCTGATAAAG	400	0.2	Müller et al. (2007)
	aggR‐R	AATACAGAATCGTCAGCATCAGC
	pic‐F	AGCCGTTTCCGCAGAAGCC	1,111	0.2	Müller et al. (2007)
	pic‐R	AAATGTCAGTGAACCGACGATTGG
	astA‐F	TGCCATCAACACAGTATATCCG	102	0.4	Müller et al. (2007)
	astA‐R	ACGGCTTTGTAGTCCTTCCAT
ETEC	LT‐F	GAACAGGAGGTTTCTGCGTTAGGTG	655	0.1	Müller et al. (2007)
	LT‐R	CTTTCAATGGCTTTTTTTTGGGAGTC
	STIa‐F	CCTCTTTTAGYCAGACARCTGAATCASTTG	157	0.4	Müller et al. (2007)
	STIa‐R	CAGGCAGGATTACAACAAAGTTCACAG
	STI‐F	TGTCTTTTTCACCTTTCGCTC	171	0.2	Müller et al. (2007)
	STI‐R	CGGTACAAGCAGGATTACAACAC
E. coli	uidA‐F	ATGCCAGTCCAGCGTTTTTGC	1,487	0.2	Müller et al. (2007)
	uidA‐R	AAAGTGTGGGTCAATAATCAGGAAGTG

STEC, Shiga toxin‐producing E. coli; EPEC, enteropathogenic E. coli; EIEC, enteroinvasive E. coli; EAEC, enteroaggregative E. coli; ETEC, enterotoxigenic E. coli.