Fig. 3. HPLC monitoring of the reaction of Zn·LHSP (35 μM) in a phosphate buffer (50 mM, pH 7.0, 298 K) with HOCl or H2O2: chromatograms (A)–(D) correspond to unreacted Zn·LHSP (A), Zn·LHSP + 3.0 eq. HOCl (B), Zn·LHSP + 1.0 eq. HOCl (C) and Zn·LHSP + 3000 eq. H2O2 (D). The star on chromatogram (D) denotes LHSP dimers with intermolecular disulfides.28 The disulfide patterns of oxidation products α, β and γ are shown on the right as identified by endopeptidase digestion and ESI-MS analyses.
