Fig. 6. (A) Oxidation of FmocMet (20 μM) by HOCl at pH 7.4 (phosphate buffer 10 mM, 298 K) monitored by HPLC. FmocMet elutes at 9.6 min and its oxidation product FmocMetO at 7.5 min. (B) Competition between FmocMet and Met at pH 7.4 monitored by HPLC. The left and right panels compare the chromatograms obtained after oxidation of FmocMet (20 μM) by HOCl in the absence (left) and presence (right) of Met (20 μM). Oxidations were performed at 298 K in a phosphate buffer (10 mM).