Table 1. Summary of ESI-MS analysis after trypsin and GluC digestion of the three LHSP bis-disulfide isomers.
| Fragment | Mass/Da | Observed m/z |
||
| Isomer α (tR = 16.8 min) | Isomer β (tR = 16.3 min) | Isomer γ (tR = 15.8 min) | ||
| YDPPK | 503.27 | 504.2 (+), 252.5 (2+) | 504.2 (+), 252.5 (2+) | 504.2 (+), 252.5 (2+) |
| C 2EC4NAAK (1 S–S) | 735.27 | 736.3 (+), 368.6 (2+) | — | — |
| VC11K + NC14QTR (1 S–S) | 966.44 | 967.5 (+), 484.2 (2+) | — | — |
| AcKC2E + NC14QTR (1 S–S) | 1038.42 | — | 1039.4 (+), 520.1 (2+) | — |
| C 2E + NC14QTR (1 S–S) | 868.31 | — | 869.3 (+), 435.1 (2+) | — |
| C 4NAAK + VC11K (1 S–S) | 851.40 | — | 852.4 (+), 426.6 (2+) | — |
| AcKC2E + VC11K (1 S–S) | 766.33 | — | — | 767.3 (+), 384.1 (2+) |
| C 2E + VC11K (1 S–S) | 596.23 | — | — | 597.2 (+), 299.1 (+) |
| C 4NAAK + NC14QTR (1 S–S) | 1123.49 | — | — | 562.7 (2+) |
| AcKC2EC4NAAK + C11K + NC14QTR (2 S–S) | 1871.81 | — | 936.9 (2+), 625.1 (3+) | 937.4 (2+), 625.1 (3+) |
| AcKC2EC4NAAK + VC11K + NC14QTR (2 S–S) | 1701.71 | — | 851.9 (2+), 568.4 (3+) | 851.9 (2+), 568.5 (3+) |
| AcKC2EC4NAAK + C11KNC14QTR (2 S–S) | 1853.80 | — | 927.9 (2+), 619.2 (3+) | — |
| C 2EC4NAAK + C11KNC14QTR (2 S–S) | 1683.69 | — | 842.8 (2+), 562.5 (3+) | — |