Figure 1.
Alignment of the actual and predicted DNA recognition sequences for VMA inteins from S.cerevisiae, DH1-1A and C.tropicalis. At the top of the figure is shown the protein sequence of the VMA1-encoded H+-ATPase in the region overlapping the intein insertion site. The position of the intein insertion site is indicated by an arrow. Base pairs that are shown on a red background in the S.cerevisiae intein– sequence are critical for DNA cleavage by PI-SceI (20). The staggered line denotes the site of cleavage by PI-SceI. In the DH1-1A and C.tropicalis intein– sequences, the base pairs shown on a green background are those that differ from the S.cerevisiae intein– sequence.
