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. 2001 Oct 15;29(20):4215–4223. doi: 10.1093/nar/29.20.4215

Figure 2.

Figure 2

Binding of the predicted endonuclease recognition sequences from S.cerevisiae, the DH1-1A intein– allele and C.tropicalis to purified PI-SceI protein determined by EMSA. Radiolabeled 219 bp substrates containing one of the different recognition sequences was incubated with increasing concentrations of PI-SceI corresponding to 3 × 10–10, 7 × 10–10, 1 × 10–9 and 3 × 10–9 M in each set of four lanes. The samples were separated by electrophoresis in a 7% polyacrylamide gel. The migration positions of unbound 219 bp fragment DNA and the lower and upper PI-SceI–DNA complexes are indicated by UB, LC and UC, respectively.