Table 1.
Summary of gRNAs targeting nox genes
| gRNA | Sequence (5′-3′) | PAM | Score | Gene ID | Chromosome nol |
|---|---|---|---|---|---|
| nox1 I | GGGAAAGGGATTAAGGTAAG | TGG | 96 | 555604 | 14 |
| nox1 II | GGTTTAGTTTTCCACGGCGC | AGG | 98 | ||
| nox2/cybb I | GGAAACTTTGCTGCAAATGA | AGG | 97 | 393386 | 11 |
| nox2/cybb II | GGATTGGTGCTTCATGGAAT | CGG | 97 | ||
| nox5 I | GGTGACCCATTCCAGCCAGC | GGG | 99 | 100149800 | 25 |
| nox5 II | GGGAGATCGCACTCTCTCGC | AGG | 96 | ||
| duox I | GGTGCTGATCCTCCGAGGGT | TGG | 95 | 565097 | 25 |
| duox II | GGCAGACTGGGACACGAGCA | CGG | 99 |
gRNA sequences are listed, along with the protospacer adjacent motif (PAM). Ideal PAM sites are NGG. Scores were assigned using design software (see Materials and Methods). Gene IDs refer to GenBank accession numbers.