Table 2.

Summary of potential off-target sites for gRNAs

gRNA	Sequence (5′-3′)	PAM	Score	Gene ID	Chromosome no.
nox1 I	AGGAAAGATATTAAGGTGAG	GAG	0.2	567212	15
nox1 II	GGTTTTGTTTTCCCAGGCTC	CAG	0	567798	2
nox2/cybb I	GCAGACTTTCCTCCAAATGA	AGG	0.5	387260	8
nox2/cybb II	GGATTGGGGCTTCGTGGAGG	AGG	0	393852	7
nox5 I	GGTCTCCCACTCCTGCCAGC	AAG	0.2	568565	20
nox5 II	GTGAGATCGCAGTCCCTCGA	AAG	0.1	368662	2
duox I	GATGCTGTTCCTCCGATGGA	CAG	0.3	563429	5
duox II	GGGGGACTGCGAAACGAGCA	GAG	0.5	368415	12

Sequences for the predicted off-target sites are listed, along with the accompanying protospacer adjacent motif (PAM). Bold characters represent mismatches. Scores were assigned using design software (see Materials and Methods). Gene IDs refer to GenBank accession numbers and the chromosome on which each sequence appears is given.