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. 2018 Jun 27;38(26):5854–5871. doi: 10.1523/JNEUROSCI.1483-16.2018

Table 4.

Efficiency of gRNAs

gRNA I
gRNA II
Mutant Total % Mutant Total %
nox1 50 55 91 46 61 75
nox2/cybb 40 60 67 49 58 84
nox5 35 52 67 50 55 91
duox 49 55 89 42 50 84

Embryos were genotyped as described in the Materials and Methods. “Total” refers to the total number of embryos that were injected and genotyped; “mutant” refers to the number of embryos found to carry mutations.