Fig. 8. (A) Increased production of ATP in MCF-7 breast cancer cells in the presence of α-TOS, T-α-TOS-NPs, and NT-α-TOS-NPs. There was no significant increase in the ATP level in normal H9C2 cardiomyocytes in the presence of α-TOS, T-α-TOS-NPs, and NT-α-TOS-NPs. (B) Relative lactate level in MCF-7 cells treated with oligomycin, α-TOS, or T/NT-α-TOS-NPs. (C) Increased ROS production in MCF-7 in presence of α-TOS, T-α-TOS-NPs, and NT-α-TOS-NPs. There was no significant increase in the ROS level in normal H9C2 cardiomyocytes in the presence of α-TOS, T-α-TOS-NPs, and NT-α-TOS-NPs. (D) H2O2 production in MCF-7 cells by an Amplex Red-hydrogen peroxide/peroxidase assay in the presence of α-TOS, NT-α-TOS-NPs, or T-α-TOS-NPs (40 μM with respect to α-TOS) without or with 100 mM malonate for 24 h. ns: non significant. ***P < 0.001; **P = 0.001–0.01.
