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. 2018 Jun 21;6:227. doi: 10.3389/fchem.2018.00227

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Copyright © 2018 Heenatigala, Yang, Bishopp, Sun, Li, Kumar, Hu, Wu, Lin, Yao, Duan and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical GUS assay of transgenic W. globosa via Agrobacterium-mediated stable transformation after the 4 weeks of selection. (A) TCS::GUS transformed W. globosa transgenic fronds (B) TCS::GUS transformed W. globosa transgenic frond showing GUS expression in both mother and daughter frond. (C,D) GUS positive daughter frond comes out from the single side pouch of the stably transformed mother frond. (E,F) Cross sections of transgenic W. globosa via Agrobacterium-mediated stable transformation after histochemical GUS assay. TCS::GUS transformed W. globosa transgenic frond showing GUS staining in both mother and daughter frond. MF, Mother Frond; DF, Daughter Frond; Scale bars (A–D) - 250 μm, (E,F) - 50 μm.