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. 2018 Jun 21;6:227. doi: 10.3389/fchem.2018.00227

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Copyright © 2018 Heenatigala, Yang, Bishopp, Sun, Li, Kumar, Hu, Wu, Lin, Yao, Duan and Hou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of the e Wolffia transformed with TCS::GUS using PCR & RT- PCR analysis. (A) Amplification of TCS element from Wolffia transformants. (B) Amplification of GUS gene from Wolffia tarnsformants. For both gels a 2,000 bp ladder is used and the sizes of the corresponding bands are indicated. Lanes 1 and 2 contain two independent TCS::GUS transgenic plants and lane 3 (marked –ve) containes genomic DNA from a wild plant (C) RT-PCR for the GUS enzyme in Wolffia transformants. An Actin gene was used as internal control. Lanes 1 and 2: putative Wolffia transformants, WT, wild-type plant.