Table 2.

Composition of the media used in stable transformation.

Media type	Composition
YEB medium	Yeast extract 6 g l−1 +Tryptone 5 g l−1 + Sucrose 5 g l−1 + MgSO4.7H2O 0.5 g l−1 + Rifampicin 20 mg l−1+ Spectinomycin 100 mg l−1 (pH-7)
Co-cultivation Medium	Liquid SH +2% Sucrose +2 mg l−1 2, 4-D + 2 mg l−1 6 BA
Resting Medium	SH + 2% Sucrose +2 mg l−1 2, 4-D + 2 mg l−1 6 BA+ Cefotaxime 300 mg l−1
Selection Medium	SH + 2% Sucrose + 0.6% Agar + Cefotaxime 300 mg l−1+ G418 40 mg l−1
Frond Induction Medium	SH + 2% Sucrose + 0.6% Agar + Cefotaxime 150 mg l−1