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(a) Sequential targeting of LNL and FNFL cassettes
| Number of clones | Number of heteroinsertion | Number of homoinsertion | Efficiency of heteroinsertion | Efficiency of homoinsertion | ||
|---|---|---|---|---|---|---|
| Eed | LNL insertion | 88 | 17 | 4 | 19.3% | 4.5% |
| FNFL insertion | 190 | 33 | 5 | 17.4% | 2.6% | |
| SRCAP | LNL insertion | 94 | 65 | 3 | 69.1% | 3.2% |
| FNFL insertion | 96 | 70 | 2 | 72.9% | 2.1% |
