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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Mol Cell. 2018 Mar 1;69(5):757–772.e7. doi: 10.1016/j.molcel.2018.01.037

Figure 1. GPS2 localizes to mitochondria and regulates mitochondrial function through neMITO gene expression.

Figure 1

(A) GPS2 and mtHSP70 immunostaining in 3T3-L1 and Hela cells.

(B) WB of 293T cells are transfected with full length GPS2 (wt), GPS2 with deletion of first 60 aa and mutation of K254,300,327 (Δ60-CtKsR), GPS2 1–155 and 1–212.

(C) Decreased respiration and oxygen consumption in 3T3-L1 GPS2-KD cells.

(D) Relative expression of representative neMITO genes by RT-qPCR in 3T3-L1 cells.

(E) Mitochondrial proteins expression by WB with relative quantification, n=3 independent blots.

(F) Mitochondrial DNA content in WT and GPS2-KD 3T3-L1 and 293T cells.

* indicate p-value<0.05; ** indicate p-value<0.01.