Fig. 1.

Fruit-specific PHY knockdown in transgenic tomato plants. (A) Constructs designed for generation of the SlPHYA^RNAi, SlPHYB1/B2^RNAi, and SlPHYB2^RNAi transgenic lines. ‘A’ indicates the SlPHYA-specific fragment of the mRNA 5′ untranslated region (UTR). ‘B1/B2’ indicates the SlPHYB1/B2-specific fragment of the mRNA 5′ UTR. ‘B2’ indicates the SlPHYB2-specific fragment of the mRNA 5′ UTR. (B) Relative SlPHY mRNA levels in leaves, and immature green (IG), mature green (MG), and breaker (Bk) stages of fruits of the SlPHYA^RNAi, SlPHYB2^RNAi, and SlPHYB1/B2^RNAi lines. The first and second fully expanded leaves from the top of 2-month-old plants were harvested. Transcript abundance was normalized against the wild-type (WT) sample. Statistically significant differences compared with the WT genotype were determined using Student’s t-test: *P<0.05. Data are means (±SE) of at least three biological replicates. (This figure is available in color at JXB online.)