Fig. 5.

Fruit-specific SlPHYA or SlPHYB2 knockdown represses carotenoid biosynthesis during tomato fruit ripening. (A) Lycopene, phytoene, phytofluene, β-carotene, lutein, and total carotenoid content in red ripe fruits. (B) Schematic representation of carotenoid biosynthetic pathway and graphs showing the transcript abundance of carotenoid biosynthesis genes in ripening fruits. Intermediate reactions are omitted. For simplicity, the mean of the three values for the transgenic lines is shown. Values for each transgenic line are presented in Supplementary Fig. S9, Supplementary Table S4. Data are means (±SE) of at least three biological replicates. Statistically significant differences compared with the wild-type (WT) sample were determined using Student’s t-test: *P<0.05. MG, mature green; Bk, breaker; RR, red ripe; MEP, methylerythritol 4-phosphate; GGDP, geranylgeranyl diphosphate; GGPS, GGDP synthase; PSY, phytoene synthase; PDS, phytoene desaturase; LCYβ, chloroplast-specific β-lycopene cyclase; CYCβ, chromoplast-specific β-lycopene cyclase. (This figure is available in color at JXB online.)