Fig. 5.

The spectral clustering-based method identifies clones with more shared mutations. (A–D) The number of shared mutations in the V segment (up to the start of the junction) was determined for the 50 largest clones (covering $\sim 30\%$ of the total reads) inferred by spectral clustering (black bars towards left), hierarchical clustering (black bars towards right) and negative controls (grey bars). Results are shown for the same four experimental datasets shown in Figure 4. Note that fewer than 50 clones are shown because some pairs of largest inferred clones did not overlap or no shared mutation was observed for either method. (E–H) The total number of shared mutations across all clones identified by the spectral (circles) and hierarchical (triangles pointing up) clustering-based methods, as well as a negative control (triangles pointing down) was determined for each subject M2–M5