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. 2001 Oct 15;29(20):e98. doi: 10.1093/nar/29.20.e98

Figure 2.

Figure 2

Recombinant human c-Fos/c-Jun binds DNA with high affinity. Increasing amounts of recombinant human c-Fos/c-Jun were added to a DNA fragment containing a single high affinity AP-1 element. The binding of c-Fos/c-Jun to the DNA fragment was analyzed by DNase I footprinting. The final concentrations of c-Fos/c-Jun heterodimer in reactions were: lane 1, 0 nM; lane 2, 0.4 nM; lane 3, 0.6 nM; lane 4, 0.8 nM; lane 5, 1.0 nM; lane 6, 1.3 nM; lane 7, 1.6 nM; lane 8, 2.1 nM; lane 9, 2.8 nM; lane 10, 3.6 nM; lane 11, 4.7 nM; lane 12, 6.1 nM; lane 13, 7.9 nM; lane 14, 10 nM; lane 15, 13 nM; lane 16, 17 nM; lane 17, 22 nM; lane 18, 29 nM. The DNA concentration was 0.5 nM. The protected region is indicated with a bracket.