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. 2001 Oct 15;29(20):e98. doi: 10.1093/nar/29.20.e98

Figure 4.

Figure 4

Recombinant c-Fos/c-Jun activates transcription in vitro. (A) Recombinant c-Fos/c-Jun activates transcription in an extract prepared from unstimulated Jurkat cell nuclei. The template used contained five AP-1 elements upstream of the E1b TATA box and the CAT gene. Transcripts were analyzed by primer extension using a CAT specific primer followed by denaturing PAGE. The concentrations of c-Fos/c-Jun in reactions were: lane 1, 0 nM; lane 2, 12 nM; lane 3, 20 nM; lane 4, 40 nM; lane 5, 0 nM. The DNA concentration was 2 nM. (B) Recombinant c-Fos/c-Jun activates transcription in a reconstituted human transcription system. The DNA template [p(AP-1)5-E1b-G-less] contained five AP-1 elements upstream of the E1b TATA box and a 377 bp G-less cassette. Transcripts were analyzed by denaturing PAGE. The concentrations of c-Fos/c-Jun in reactions were: lane 1, 0 nM; lane 2, 2.4 nM; lane 3, 4.8 nM; lane 4, 7.2 nM; lane 5, 9.6 nM. The DNA concentration was 0.8 nM.