Fig. 3.

Double-mutant analysis of ply2 and smax1. (A) Phytochrome B (phyB)-dependent germination of the wild-type (WT, Col), ply2, smax1-3, and ply2smax1-3 double-mutant. After treatment with far-red light for 10 min, the seeds were then either irradiated (phyB_on) or not (phyB_off) with red light for 10 min, and then kept in darkness for 4 d. Mean values (±SD) of germination rates were determined from three experimental replicates with at least 50 seeds per plate. (B) Hypocotyl elongation assay. The images show representative 6-d-old seedlings of each genotype grown on half-strength MS-SUC (0.5%) media under short-day conditions (10/14 h light/dark); scale bar, 5 mm. The graph shows the mean (±SD) hypocotyl length of the seedlings (n=15–18). Different letters indicate significant differences at P<0.01, analysed by one-way ANOVA with a post hoc Tukey HSD test. (C) Leaf morphology of 30-d-old plants. The plants were grown under short-day conditions (10/14 h light/dark); scale bars, 10 mm. (D) Primary dormancy assay. Freshly harvested seeds of each genotype were sown on aqueous media and incubated under continuous light conditions. Mean values (±SD) of germination rate were determined at different times after sowing for three experimental replicates with at least 50 seeds per plate.