Fig. 2.

Absolute quantification of 13-62 transcripts in Craterostigma plantagineum, Lindernia brevidens, and Lindernia subracemosa by RT–qPCR analysis. cDNA was prepared from total RNA isolated from leaves of untreated, partially dehydrated (relative water content 50–60%), and late dehydrated (relative water content 30–40%) plants and amplified using 13-62-specific primers. Transcript copy numbers were calculated from three different biological replicates (mean +SD). Different letters above bars denote statistically significant differences within each group of samples (P<0.05; one-way ANOVA).