Fig. 1.

Arabidopsis PLDα1 negatively modulates while PLDδ positively modulates post-penetration resistance against well-adapted powdery mildew Gc UCSC1. (A) Representative images of Arabidopsis leaves of the indicated genotypes infected with Gc UCSC1 at 12 dpi. Note, pldα1 and pldα1δ were less susceptible while pldδ was more susceptible than Col-0. (B) Quantification of spore production in the indicated genotypes at 10 dpi normalized to leaf FW. Data represent the mean ±SEM of three samples (n=3, four leaves each) from one experiment, which was repeated three times with similar results. (C) Representative microscopic images of single colonies of Gc UCSC1 on leaves of the indicated genotypes at 4 dpi. Fungal structures were stained by trypan blue. Scale bars=200 μm. (D) Total number of conidiophores per colony on leaves of the indicated genotypes at 4 dpi. The boxplot shows combined data from three independent experiments (at least 20 colonies were counted for each genotype per experiment). The bold line within the box represents the median. The bottom and top edge of the box represent the first and third quartile, respectively. Ends of whiskers represent the minimum and maximum of data points. Gray dots represent outliers. Different lower case letters indicate statistically different groups (P<0.01) as determined by multiple comparisons using one-way ANOVA, followed by Tukey’s HSD test.