Fig. 1.

Changes in movement of CF after nutrient stress. (A) Distance of CF transport (from the root tip shootwards) in roots grown for 24 h under the treatment conditions indicated in the figure. For this assay, CFDA was micropipetted to the root tip and then incubated at room temperature for 5 min prior to imaging. Distance of movement was then measured as described in the Materials and methods on modified images using ImageJ. Green boxes indicate an increase in movement; pink indicates a decrease. A significant difference relative to control images is also indicated by asterisks (Wilcoxon rank sum test; P<0.05, n>24). (B, B'). Representative images of CF after a color threshold has been applied using ImageJ under (B) control or (B') 600 µM Fe. The red arrows indicate the direction and approximate extent of CF movement.