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. 2018 Jun 13;69(15):3715–3728. doi: 10.1093/jxb/ery171

Fig. 5.

Fig. 5.

cals5 and cals12 seedlings have altered responses to excess iron. (A–D) Aniline blue staining of callose in (A and A') the wild type, (B and B') cals5-2, and (C and C') cals12-1 under (A–C) control conditions, and (A'–C') 24 h after treatment with 600 µM Fe. (D) Quantification of callose levels in cals5-2 and cals12-1 roots after 24 h of growth on control medium or medium containing 600 μM Fe. Asterisks indicate P<0.05, two-tailed t-test, n>19. (E–G') Treatment of cals5 and cals12 seedlings with 600 µM Fe has no effect on the movement of GFP. GFP localization in 6-day-old wild-type, cals5, and cals12 seedlings expressing SUC2:GFP 24 h after transfer to (E–G) standard MS (control) medium or (E'–G') medium containing 600 µM iron. QC:P ratios (as shown in Fig. 2) for (F( and (F') are 0.49 and 0.51, respectively, and 0.63 and 0.64 for (G) and (G'), respectively. Scale bars in A–G'=100 µm.