Fig. 1.

Integration of Nicotiana tabacum AQP1 into the tobacco chloroplast genome. (A) Map of the wild-type (WT) and AQP1-transformed plastid (pt) genomes. The transgenes were targeted to the intergenic region between trnI and trnA. The selectable marker gene aadA (encoding aminoglycoside 3ʹ-adenylyltransferase) was driven by the 16S ribosomal RNA operon promoter (Prrn). AQP1 was driven by the psbA promoter and 5ʹ-untranslated region (PpsbA). Arrows within boxes show the direction of transcription. Numbers below each ptDNA indicate the predicted size of hybridizing fragments when total DNA was digested with BamHI. A 0.8 kb fragment of the targeting region for homologous recombination was used as a probe (P1) for Southern blot analysis. TpsbA, 3ʹ-untranslated region of the psbA gene. (B) Southern blot analysis of two independent lines (1 and 2) for each transformation cassette.